The FLAG tag is commonly known as the DYKDDDDK epitope tag and thus the anti-DYKDDDDK Antibody can be used for the detection of FLAG-tagged proteins. This monoclonal antibody recognizes the well-known FLAG epitope tag and detects N- or C-terminally tagged FLAG fusion proteins on Western blots.
The FLAG peptide is used as an N- or C- terminal epitope tag in many fusion proteins because it is small and therefore unlikely to alter the biochemical properties or localization of the protein to which it is fused.
Beads for Purification of FLAG-Tagged Proteins
Anti-DYKDDDDK Beads consist of monoclonal anti-DYKDDDDK antibody crosslinked to agarose beads. These beads may be used to purify or immunoprecipitate FLAG-tagged fusion proteins from cell lysates.