The BIC method for use with Brevibacillus choshinensis allows PCR product to be mixed with pBIC Vector DNA. Both insert and vector are designed to include 15-bp homologous overhangs to allow homologous recombination in vivo, yielding the desired construct. Four different pBIC vectors are included, each with a different type of secretion signal sequence. This allows rapid generation of a suite of constructs to allow screening for the expression plasmid that allows optimal secretory expression. The pBIC system enables creation of three different types of constructs for Brevibacillus choshinensis expression: 1) His-tag secreted protein expression, 2) untagged secreted protein expression, and 3) intracellular protein expression.