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Products >  Protein_Expression_and_Purification >  Antibody_Purification >  Capturem_Protein_A >  Miniprep_Kit

Rapid Antibody Purification Kits—Capturem Protein A Miniprep

Capturem Protein A Miniprep columns provide rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. These miniprep columns combine the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. These columns are convenient, powerful, and efficient tools for small-scale antibody purification, and offer the following advantages:

  • Fast and convenient: purify antibodies in 5 minutes at room temperature
  • High quality: short residence times reduce the possibility of aggregation or loss of activity
  • High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
  • Flexible: purify a wide range of antibodies

Protein A is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the FC region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, and human antibodies. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, or 96-well plates.

Capturem Protein A Miniprep Columns

Capturem Protein A Miniprep consists of single-use, disposable spin columns that yield up to 300 µg of purified antibody per column, depending upon species and antibody isotype. These miniprep columns are suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources. Capturem Protein A Miniprep columns can purify antibodies with a 5-minute protocol at room temperature, and at a binding capacity of up to 75 mg/ml or more, far exceeding the capacity of resin-bound Protein A columns.

Where resin columns fall short, Capturem Protein A Minipreps yield highly pure monoclonal and polyclonal antibodies in just 5 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of protein aggregation or loss of activity, giving downstream applications a higher probability of success.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • No-waiting workflow: 5-minute, room-temperature protocol
  • High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
  • High concentration: extremely small elution volumes yield highly concentrated antibodies
  • High quality: short residence times reduce the possibility of aggregation or loss of activity
  • Versatile: purify a wide range of antibodies
  • Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

Applications

  • Antibody purification
  • Antibody screening
  • Immunoprecipitation (view data)

Antibody Affinities of Capturem Protein A Miniprep

Species Affinity (µg/column)*
Goat100–150
Horse>150
Human>150
Mouse100–150
Rabbit50–100
Rat>150
Sheep>150

* Whole serum from various species was diluted in a phosphate-based binding buffer (pH 8.0) and bound on Capturem Protein A Miniprep columns. The amount of purified antibody obtained upon elution was determined by measuring absorbance at 280 nm.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
635717 Capturem™ Protein A Miniprep 12 Purifications $189.00 License Statements
 

 

 
 

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