Capturem Protein A Maxiprep columns provide rapid, high-quality purification of monoclonal and polyclonal antibodies. These maxiprep columns combine the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. These columns are convenient, powerful, and efficient tools for antibody purification, and offer the following advantages:
- Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
- High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
- High quality: short residence times reduce the possibility of aggregation or loss of activity
- Flexible: purify a wide range of antibodies
Protein A is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, and human. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, or 96-well plates.
Capturem Protein A Maxiprep Columns
Capturem Protein A Maxiprep consists of single-use, disposable spin columns that yield up to 2.5 mg of purified antibody per column, depending upon species and antibody isotype. These maxiprep columns are suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources. Capturem Protein A Maxiprep columns can purify antibodies with a 15-minute protocol at room temperature that requires no incubation step, and at a binding capacity of up to 75 mg/ml or more, far exceeding the capacity of resin-bound Protein A columns. Capturem Protein A technology is also available in miniprep columns and 96-well plates that yield highly concentrated antibodies in minutes and allow rapid, high-throughput antibody screening.
Where resin columns fall short, Capturem Protein A Maxipreps yield highly pure monoclonal and polyclonal antibodies in just 15 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of antibody aggregation or loss of activity, giving downstream applications a higher probability of success.