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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Antibody_Purification >  Capturem_Protein_A >  96-Well_Plate

High-Throughput Antibody Purification—Capturem Protein A 96

Capturem Protein A 96 is a 96-well plate that provides rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. This 96-well plate combines the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification in a high-throughput format

Screen your antibodies with a purification method that is:

  • High-throughput: purify 96 antibody samples simultaneously
  • Fast and convenient: room-temperature protocol, complete in under 15 minutes
  • High quality: short residence times reduce the possibility of aggregation or loss of activity
  • High purity: small bed volumes in plate wells trap few contaminants and allow large-volume washes
  • Flexible: purify a wide range of antibodies

Protein A is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, and human. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, and 96-well plates.

Capturem Protein A 96-Well Plate

Capturem Protein A 96 consists of a single-use, disposable 96-well plate that yields up to 300 µg of purified antibody per well, depending upon species and antibody isotype. This 96-well plate is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources. Capturem Protein A 96 can purify antibodies in under 15 minutes at room temperature, and at a binding capacity of up to 75 mg/ml, far exceeds the capacity of resin-bound Protein A columns. Starting materials, specific antibody affinities, yields, purification times, and binding capacities of individual wells are comparable to individual Capturem Protein A Miniprep columns.

Where resin columns fall short, Capturem Protein A 96 yields highly pure monoclonal and polyclonal antibodies in just 5 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of antibody aggregation or loss of activity, giving downstream applications a higher probability of success.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Purify 96 antibody samples simultaneously: use either vacuum filtration or centrifugation
  • No-waiting workflow: 15-minute, room-temperature protocol
  • High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
  • High concentration: extremely small elution volumes yield highly concentrated antibodies
  • High quality: short residence times reduce the possibility of aggregation or loss of activity
  • Versatile: purify a wide range of antibodies

Applications

  • Antibody purification
  • Antibody screening
  • Immunoprecipitation and co-immunoprecipitation

Antibody Affinities of Capturem Protein A Miniprep

Species Affinity (µg/column)*
Goat100–150
Horse>150
Human>150
Mouse100–150
Rabbit50–100
Rat>150
Sheep>150

* Whole serum from various species was diluted in a phosphate-based binding buffer (pH 8.0) and bound on Capturem Protein A Miniprep columns. The amount of purified antibody obtained upon elution was determined by measuring absorbance at 280 nm.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Offers Cat. # Product Contents Size Price License Units Select
635716 Capturem™ Protein A 96 96 Purifications $415.00 License Statements
 

 

 
 

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