SYBR Advantage qPCR Premix is a convenient, ready-to-use 2X-concentrated master mix for real-time PCR that contains full-length Taq polymerase with hot start antibody and SYBR Green I. It is specially designed for real-time PCR employing the dye intercalator method. This qPCR master mix combines the high performance of our enzyme for hot start PCR utilizing Taq antibody and a newly developed buffer, to provide superior specificity, increased amplification efficiency, and excellent performance in high-speed, real-time PCR. Use of the SYBR Advantage qPCR Premix enables you to carry out successful real-time PCR with high sensitivity, broad dynamic range, and accurate quantification.
SYBR Advantage qPCR Premix is supplied separately with ROX Reference Dye LSR and ROX Reference Dye LMP, which allow you to normalize the fluorescence signal between reactions on instruments that are equipped with this option. Recent studies have shown that the SYBR Advantage qPCR Premix is superior to corresponding reagents offered by three leading competitors in the qPCR arena, in terms of reaction specificity, amplification efficiency, and sensitivity.
More Specific than Competitor R
Competitor R’s real-time PCR enzyme showed poor reaction specificity when compared to the SYBR Advantage qPCR Premix. This was evidenced by multiple peaks in the melting curve for Competitor R's enzyme, particularly when low-copy-number templates were amplified.
More Efficient than Competitor A
Competitor A's SYBR qPCR master mix showed a lower amplification efficiency than that of the SYBR Advantage qPCR Premix. This was indicated by Ct values that were shifted to the right.
More Specific than Competitor I
The SYBR Advantage qPCR Premix demonstrated superior reaction specificity when compared to Competitor I's SYBR qPCR master mix. This was indicated by tighter peaks in the melting curve for the Clontech enzyme.
Twice as Fast as Competitor A
Excellent results were obtained in about half the time (50 min versus 90 min) when using the SYBR Advantage qPCR Premix as opposed to Competitor A's TaqMan master mix (data not shown). These experiments were carried out with the Applied Biosystems 7500 Real-Time PCR System.
Clearly, the Clontech SYBR Advantage qPCR Premix outperforms much of the competition in terms of specificity (melting curves indicate the presence of a single product), efficiency, and sensitivity (lower Ct values in the amplification plots). Moreover, when using the Clontech SYBR Advantage qPCR Premix, qPCR analysis can be accomplished in nearly half of the time required for other qPCR chemistries.
Compatible cyclers: SmartCycler, LightCycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems StepOne and StepOnePlus, 7300/7500, iCycler, Opticon, Stratagene MX 3000P and MX3005P, and QIAGEN Rotor-Gene.