Taq polymerase is the premier choice for qPCR applications, whether real time or endpoint. It displays a robustness and sensitivity that ensure the optimal amplification of a variety of targets—including rare ones—from both cDNA and genomic templates, in both single and multiplex reaction formats. This is especially important when amplifying low-copy-number targets or complex templates.
Hot Start Antibody Delivers High Specificity
By blocking polymerase activity during the initial PCR reaction set-up, our hot start system prevents the formation of primer dimers and other artifacts of low-level synthesis that can arise prior to thermal cycling. Our antibody-mediated hot start system also allows for higher specificity compared to other traditional Taq-based systems. This is particularly important when multiplexing two or more targets in the same reaction, or when quantifying with homogeneous fluorescence detection systems, such as SYBR Green I, that bind to all double-stranded DNA products (including primer-dimers and non-specific amplicons).
Ready-to-use qPCR master mix that contains full-length Taq polymerase and SYBR Green I is also available.