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PCR, RT-PCR, Real-Time qPCR


Products >  PCR_RT-PCR_Real-Time_qPCR >  Real-Time_qPCR_Reagents >  QTaq_for_Real-Time_qPCR

QTaq DNA Polymerase for Real-Time qPCR

QTaq DNA Polymerase Mix contains a robust, full-length Taq DNA Polymerase designed to keep you at the forefront of quantitative PCR (qPCR) technology. The mix includes a built-in hot start antibody, which guarantees increased specificity in each PCR reaction. Optimized for use with most qPCR detection methods and applications, our polymerase mix provides high sensitivity and reproducibility for qPCR and qRT-PCR (quantitative reverse transcriptase PCR) assays. For convenience, the QTaq PCR Buffer includes dNTPs, Mg2+, and ROX passive reference dye.

Maximum Sensitivity

Taq polymerase is the premier choice for qPCR applications, whether real time or endpoint. It displays a robustness and sensitivity that ensure the optimal amplification of a variety of targets—including rare ones—from both cDNA and genomic templates, in both single and multiplex reaction formats. This is especially important when amplifying low-copy-number targets or complex templates.

Hot Start Antibody Delivers High Specificity

By blocking polymerase activity during the initial PCR reaction set-up, our hot start system prevents the formation of primer dimers and other artifacts of low-level synthesis that can arise prior to thermal cycling. Our antibody-mediated hot start system also allows for higher specificity compared to other traditional Taq-based systems. This is particularly important when multiplexing two or more targets in the same reaction, or when quantifying with homogeneous fluorescence detection systems, such as SYBR Green I, that bind to all double-stranded DNA products (including primer-dimers and non-specific amplicons).

Ready-to-use qPCR master mix that contains full-length Taq polymerase and SYBR Green I is also available.

  At-A-Glance   Documents   Images & Data

Features

  • Provide superior sensitivity and high yields
  • Allow the accurate quantitation of gene expression from a variety of targets, including rare ones
  • Provided in an easy-to-use master mix format that allows you to use your favorite detection chemistry (SYBR or TaqMan)
  • Buffer contains ROX passive reference dye

Applications

  • Primary gene expression analysis
  • Confirmation of microarray data
  • Viral load detection
  • Assessment of knockout efficiency
  • Assessment of transgene copy number
  • Allelic discrimination

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
639652 QTaq™ DNA Polymerase Mix 10 x 200 Rxns $2,100.00 License Statements
639655 QTaq™ DNA Polymerase Mix 2,000 Rxns $1,900.00 License Statements
 

 

 
 

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