Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that includes a proofreading DNA polymerase and our highly robust, hot start enzyme Titanium Taq DNA Polymerase. The combination provides a proven balance of high yield and high fidelity for cDNA amplification and library construction that requires minimal optimization. Advantage 2 is an extremely versatile polymerase mix that can readily amplify a wide range of DNA templates, including long PCR templates up to 18 kb. With three times higher fidelity than regular Taq, Advantage 2 polymerase mix is also perfect for PCR applications requiring accuracy, such as RACE PCR, cDNA amplification, library construction, and PCR Subtraction. Advantage 2 Polymerase Mix is highly recommended for applications downstream of SMART(er) cDNA synthesis.
The Advantage 2 Polymerase Mix consists of:
- Titanium Taq DNA Polymerase—a robust, sensitive enzyme that provides higher yields than Taq, and contains TaqStart Antibody for convenient hot start PCR.
- Proofreading polymerase—for longer PCR products and increased fidelity.
The Advantage 2 Polymerase Mix is available by itself (with an optimized buffer) or in a complete PCR kit that includes dNTPs and PCR controls.