The PCR Intro Pack for PCR troubleshooting provides a selection of four PCR polymerases to solve most PCR problems: Titanium Taq DNA Polymerase, Advantage 2 Polymerase Mix, Advantage HD Polymerase Mix, and Advantage Genomic LA Polymerase Mix. This PCR troubleshooting 4-pack provides an economical way to determine which polymerase works best for your specific application. The PCR Intro Pack also includes our Advantage UltraPure PCR Deoxynucleotide Mix, a premixed aqueous solution containing 10 mM of each dNTP. The PCR Intro Pack provides enough reagents to allow 40 PCR reactions per enzyme.
Titanium Taq DNA Polymerase is a premium enzyme blend containing an N-terminal deletion mutant of Taq DNA Polymerase and TaqStart, our premier hot start antibody. Titanium Taq is a robust enzyme that lacks 5'–3' exonuclease activity, accommodates a wider range of MgCl2 concentrations, and exhibits greater thermostability and solubility than the wild-type enzyme. The addition of TaqStart ensures reduced background and exceptional sensitivity.
Advantage 2 Polymerase Mix combines Titanium Taq DNA Polymerase with a small amount of proofreading enzyme. This enzyme blend provides the same benefits as Titanium Taq, plus improved fidelity and increased product length.
Advantage HD Polymerase Mix is a novel, “high definition” DNA polymerase that provides exceptionally high accuracy and efficiency over a broad range of PCR conditions. The enzyme’s superior performance is due in part to the presence of a robust 3'–5' exonuclease (proofreading) activity and high priming efficiency. High specificity is achieved by the inclusion of a hot start antibody. When used with its optimized buffer, Advantage HD provides the high fidelity, sensitivity and specificity required for critical applications.
Advantage Genomic LA Polymerase Mix exhibits high efficiency and increased fidelity on extremely long (~30 kb) targets. This enzyme mix excels at “long and accurate” amplification of genomic DNA. For amplification of GC-rich or complex genomic templates, we recommend Advantage GC Genomic LA Polymerase Mix, which contains the same enzyme as the Advantage Genomic LA Polymerase Mix, but is optimized for more complex templates through the use of a specially formulated buffer.