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Products >  PCR_RT-PCR_Real-Time_qPCR >  PCR_Reagents_and_Accessories >  Advantage_cDNA_Polymerase

Advantage cDNA Polymerase & PCR Kits

Advantage cDNA Polymerase was designed for improved performance and reliability in the amplification of cDNA targets of up to 10 kb. Benefits over native Taq DNA Polymerase include greater efficiency and sensitivity, amplification of longer products, built-in hot start, and increased fidelity.

Advantage cDNA PCR Kits contain Advantage cDNA Polymerase Mix—an enzyme blend consisting of an N-terminal deletion mutant of Taq DNA Polymerase (1, 2), a proofreading enzyme, and TaqStart Antibody for automatic hot start PCR. The kits also come with an optimized buffer, dNTP mix, and control primers and template.

Our Advantage cDNA products have been optimized to produce high yields and long reads. Compared to regular Taq polymerase, Advantage cDNA Polymerase Mix provides more efficient amplification over a wide range of template concentrations. In addition, the optimal range of Mg2+ concentration is broader for Advantage cDNA Polymerase Mix than it is for most other enzymes, eliminating the need to spend time optimizing reaction conditions.

This enzyme mix has also been optimized for high fidelity PCR in our Advantage HF PCR Kits, and for complex templates in our Advantage GC cDNA Polymerase Mixes and Kits.

At-A-Glance Documents Images & Data

Features

  • Exhibit high efficiency and sensitivity
  • Amplify up to 5 kb from genomic DNA and up to 10 kb from cDNA
  • Built-in hot start for higher specificity and lower background
  • Optimized for applications involving cDNA

Applications

  • Long and accurate PCR
  • cDNA PCR
  • RACE
  • Preparative PCR
  • Hot start PCR
  • GC-rich PCR (GC)
  • High-fidelity PCR (HF)

References

  1. Barnes, W. M. (1992) Gene 112(1):29-35.
  2. Barnes, W. M. (1994) Proc. Natl. Acad. Sci. USA 91(6):2216-2220.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
639135 10X Advantage® cDNA PCR Buffer 10 mL $148.00  
639115 Advantage® GC cDNA PCR Kit 100 Rxns $525.00 License Statements
639116 Advantage® GC cDNA PCR Kit 10 Rxns $130.00 License Statements
639112 Advantage® GC cDNA Polymerase Mix 100 Rxns $315.00 License Statements
639101 Advantage® cDNA PCR Kit 100 Rxns $485.00 License Statements
639105 Advantage® cDNA Polymerase Mix 100 Rxns $290.00 License Statements
 

 

 

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