Resin-Based Enzyme Removal from DNA & RNA Solutions—QuickClean Enzyme Removal Resin
QuickClean Enzyme Removal Resin is a solid-phase matrix for phenol-free removal of proteins from aqueous solutions of single- or double-stranded nucleic acids used for quick, phenol-free deproteinization of DNA and RNA samples. QuickClean efficiently removes enzymes and other proteins from DNA or RNA samples in a brief vortex and spin step. No hazardous phenol extractions or time-consuming ethanol precipitations are required. The simple protocol can be performed in less than five minutes, and the resin’s blue color makes it easier to remove.
QuickClean Enzyme Removal Resin may be used to remove restriction enzymes, ligases, Taq DNA polymerase, RNase, and DNase after reactions with DNA or RNA to prevent those enzymes from interfering with subsequent manipulations. The resin can also be used to deproteinize DNA and RNA before electrophoresis on agarose gels. This will improve the resolution and clarity of bands when preparing for blot hybridization. If bands are to be cut out of the gel, deproteinization before electrophoresis will eliminate enzymes that may comigrate with the DNA fragments and coelute from the gel slices.