Clontech
login | register | order now | view cart | feedback
Products
PCR, RT-PCR, Real-Time qPCR


Products >  PCR_RT-PCR_Real-Time_qPCR >  PCR_Cleanup >  Gel_Filtration

Fractionation—Spin Columns—CHROMA SPIN Columns

CHROMA SPIN Columns are spin columns packed with gel filtration resin to rapidly purify and size select nucleic acid samples. The columns can be used to purify single- or double-stranded DNA or RNA from contaminants such as salts, solvents, enzymes, or proteins. Molecules larger than the particular matrix pore size are eluted out of the column while smaller molecules are retained inside the column matrix. As a result, these columns are ideal for size fractionation of libraries or for removal of primers.

Available in Three Different Sterile Buffers

  • TE buffer: ideal for routine DNA applications
  • STE (0.1 M NaCl + TE) buffer: used for applications requiring a higher salt concentration to prevent ionic interaction between molecules
  • DEPC-treated water (+ 0.1 mM EDTA): useful for RNA purification and those applications requiring a completely nuclease-free environment

Choose from Six Column Pore Sizes

CHROMA SPIN-10, -30, -100, -200, -400, and -1000. All columns, matrices, and buffers have been autoclaved to destroy nucleases. For optimal recovery and purification, there should be at least a threefold difference between the size of the desired nucleic acid molecules and the size of the contaminants.

At-A-Glance Documents Images & Data

Features

  • Adaptable—one system for any application
  • Efficient—purify samples in 10 minutes
  • Simple—apply sample and spin

Applications

  • Nucleic acid purification
  • Removal of contaminants
  • Buffer exchange
  • Library construction
  • Microarray spotting
  • PCR
  • Cloning

Principal CHROMA SPIN Column Applications

CHROMA SPIN Series

Purify oligos/primers after labeling reactions (bases)

DNA/RNA lengths (bases)

Remove primers/linkers digestion fragments (bases)

Remove proteins/enzymes (kDa)

CHROMA SPIN-10

>15

CHROMA SPIN-30

>35

<9

<30

CHROMA SPIN-100

>140

<30

<250

CHROMA SPIN-200

>300

<50

<1,000

CHROMA SPIN-400

>600

<100

<8,000

CHROMA SPIN-1000

>1,350

<300

all sizes

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
636055 CHROMA SPIN™+STE-10 Columns 20 Each $207.00
636056 CHROMA SPIN™+STE-10 Columns 50 Each $399.00
636060 CHROMA SPIN™+STE-100 Columns 20 Each $207.00
636061 CHROMA SPIN™+STE-100 Columns 50 Each $412.00
636058 CHROMA SPIN™+STE-30 Columns 50 Each $399.00
636066 CHROMA SPIN™+TE-10 Columns 50 Each $399.00
636072 CHROMA SPIN™+TE-100 Columns 20 Each $221.00
636073 CHROMA SPIN™+TE-100 Columns 50 Each $399.00
636079 CHROMA SPIN™+TE-1000 Columns 50 Each $399.00
636082 CHROMA SPIN™+TE-200 Columns 50 Each $399.00
636069 CHROMA SPIN™+TE-30 Columns 50 Each $399.00
636076 CHROMA SPIN™+TE-400 Columns 50 Each $399.00
636089 CHROMA SPIN™-100+DEPC-H2O Columns 20 Each $227.00
636090 CHROMA SPIN™-100+DEPC-H2O Columns 50 Each $427.00
636093 CHROMA SPIN™-1000+DEPC-H2O Columns 20 Each $223.00
636094 CHROMA SPIN™-1000+DEPC-H2O Columns 50 Each $427.00
636096 CHROMA SPIN™-200+DEPC-H2O Columns 20 Each $223.00
636087 CHROMA SPIN™-30+DEPC-H2O Columns 50 Each $427.00
 

 

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions