The Diversify PCR Random Mutagenesis Kit employs a nontoxic method for mutagenizing sequences of up to 4.0 kb in length. The kit broadly distributes all possible mutations without creating hot spots.
The Diversify Method
By allowing you to manipulate mutagenic conditions, the Diversify PCR Random Mutagenesis Kit provides optimal mutagenesis of sequences over an exceptionally broad size range. Select mutation rates from two to eight mutations per 1,000 bp simply by varying the amounts of two key reagents—manganese (Mn2+) and dGTP (1, 2).
The Diversify method utilizes Clontech's Titanium Taq PCR System to reliably amplify DNA fragments of up to 4.0 kb in length. This extended amplification range makes the Diversify Kit ideal for mutating operons, plasmids, and sequences corresponding to large proteins, in addition to short DNA fragments. The kit also contains a rapid control reaction that allows the relative comparison of mutation rates in the control fragment in just two hours following PCR.
The Diversify PCR Random Mutagenesis Kit includes the Titanium Taq Polymerase Mix and all of the reagents and buffers needed for mutagenesis.