Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
PCR, RT-PCR, Real-Time qPCR


Products >  PCR_RT-PCR_Real-Time_qPCR >  Mutagenesis_Reagents >  Diversify_Random_Mutagenesis_Kit

PCR Random Mutagenesis Kit—Diversify

The Diversify PCR Random Mutagenesis Kit employs a nontoxic method for mutagenizing sequences of up to 4.0 kb in length. The kit broadly distributes all possible mutations without creating hot spots.

The Diversify Method

By allowing you to manipulate mutagenic conditions, the Diversify PCR Random Mutagenesis Kit provides optimal mutagenesis of sequences over an exceptionally broad size range. Select mutation rates from two to eight mutations per 1,000 bp simply by varying the amounts of two key reagents—manganese (Mn2+) and dGTP (1, 2).

The Diversify method utilizes Clontech's Titanium Taq PCR System to reliably amplify DNA fragments of up to 4.0 kb in length. This extended amplification range makes the Diversify Kit ideal for mutating operons, plasmids, and sequences corresponding to large proteins, in addition to short DNA fragments. The kit also contains a rapid control reaction that allows the relative comparison of mutation rates in the control fragment in just two hours following PCR.

The Diversify PCR Random Mutagenesis Kit includes the Titanium Taq Polymerase Mix and all of the reagents and buffers needed for mutagenesis.

  At-A-Glance   Documents   Images & Data

Features

  • Controlled, random mutagenesis for investigating protein function
  • Precise control of mutation rate
  • No mutational hot spots
  • Obtain all possible base substitutions

Applications

  • Random mutagenesis

References

  1. Leung, D. W. et al. (1989) Technique 1:11–15.
  2. Cadwell, R. C. & Joyce, G. F. (1992) PCR Methods Appl. 2(1):28–33.
  3. Mo, J.-Y., et al. (1991) J. Mol. Biol. 222(4):925–936.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
630703 Diversify™ PCR Random Mutagenesis Kit 30 Rxns $602.00 License Statements
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions