RNA to cDNA EcoDry Premix is a convenient, lyophilized master mix for reverse transcription PCR (RT-PCR). This format allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. Each tube of premix contains reverse transcriptase and all the other components needed for RT-PCR. Simply add PCR-grade water along with your template, and you're ready to go.
A Premium Reverse Transcriptase
RNA to cDNA EcoDry Premix features our high-performance, ultra-pure SMART MMLV Reverse Transcriptase. SMART MMLV Reverse Transcriptase allows the synthesis of high-quality first-strand cDNA of up to 11.7 kb, from virtually any transcript.
Convenient Room-Temperature Storage
The EcoDry RT-PCR kit is ready-to-use. The simplified protocol has fewer steps, which saves you time and helps avoid pipetting errors. Additionally, the single-use design vastly reduces the risk of contamination. As with all EcoDry products, the premix can be stored at room temperature for months, without altering the performance of the reverse transcriptase or other components. This unprecedented convenience is especially useful in fieldwork, where access to refrigeration is limited.
Maximum Flexibility and Ease-of-Use
The RT-PCR premix is packaged individually, and sold in 8-well tube-strips with optically clear sealing caps (see Data & Images tab). Use a single tube, the entire strip, or several strips for larger experiments. Each tube contains a lyophilized master mix complete with SMART MMLV Reverse Transcriptase, dNTPs, and your choice of oligo(dT)18 primers, random hexamer primers, or a combination of both. Note: Double-primed refers to a mixture of oligo-(dT) and random hexamer primers.
The EcoDry lyophilized RT-PCR kit is room-temperature-stable. As a result it is shipped with minimal packaging, reducing your contribution of plastics and styrofoam to landfills. Additionally this eco-friendly format eliminates the need for refrigeration, further lowering environmental impact!