Advantage GC 2 Polymerase Mix is an excellent choice for amplifying complex, GC-rich templates, such as the 5' ends of many mammalian cDNAs, that cannot be amplified using conventional PCR techniques. Advantage GC 2 includes Advantage 2 Polymerase Mix, our proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two proven additives allowing amplification of sequences that are up to 90% GC with minimal buffer optimization.
When used together, DMSO and GC-Melt reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques. The Advantage GC 2 Polymerase Mix and PCR Kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix (Cat. No. 639153).