Titanium Taq DNA Polymerase lacks the 5'-exonuclease activity of wild-type Taq, which makes it more robust than other Taq polymerases and allows it to amplify highly complex DNA mixes. This novel enzyme also contains carefully engineered amino acid substitutions that increase its solubility, making it the most sensitive PCR polymerase available (2).
Major Advantages Over Other DNA Polymerases
Titanium Taq allows you to:
- Amplify your target using fewer PCR cycles while reducing background (see Images & Data tab)
- Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of magnesium concentrations (see Images & Data tab)
- Amplify rare targets
- Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA. Targets of up to 4 kb can be readily amplified when the template is a moderately abundant cDNA or a less complex genomic DNA
Integrated Hot Start for Higher Specificity
Titanium Taq comes preblended with TaqStart Antibody. This hot start antibody, which is quickly inactivated at the onset of thermal cycling, blocks polymerase activity during the PCR reaction set-up, preventing the formation of primer-dimers and other artifacts prior to thermal cycling. This integrated hot start feature allows for higher specificity than other traditional Taq-based methods. It is especially useful for multiplex PCR because it limits the number of mispriming events caused by multiple primer sets, which can result in spurious PCR products or smeared gel bands.
Ready-to-use lyophilized PCR master mixes featuring Titanium Taq DNA Polymerase are also available.