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O-157 (Verocytotoxin Genes) PCR Screening Set

Takara's O-157 (Verocytotoxin Genes) PCR Screening Kit enables simple detection of the genes for Verocytotoxin Type 1 and Type 2 (171 bp) by PCR. This kit contains all the reagents required to perform this PCR. The solution includes TaKaRa Ex Taq, which gives high yields, high efficiency, and allows highly sensitive detection in less time than conventional Taq Polymerase. All that needs to be added is sample template DNA.

Enterohemorrhagic E. coli (EHEC) strains, including the O157:H7 serotype, produce a verocytotoxin which causes hemorrhagic colitis in susceptible mammalian hosts. When detecting EHEC, it is important to quickly and accurately discriminate verocytotoxin-producing E. coli strains from nonpathogenic strains.

  At-A-Glance   Documents


  • Detection of genes of Verocytotoxin Type 1 and Type 2 by PCR from Enterohemorrhagic E. coli (EHEC) strains, such as E. coli O157:H7



EVC-1 primer (19 pmol/µL) 2 x 53 µL
EVC-2 primer (19 pmol/µL) 2 x 53 µL
TaKaRa Ex Taq DNA Polymerase (5 U/µL)50 µL
10X Ex Taq buffer1 mL
dNTP Mixture (ea. 2.5 mM)800 µL
Control Template EC3 (0.1 ng/µL)10 µL




  1. Takao, T. et al. (1988) Microb. Pathog. 5:357-69.
  2. Jackson, M.P. et al. (1987) FES Microbio. Lett. 44:109-4.
  3. Ito, H. et al. (1990) Microb. Pathog. 8:47-60.
  4. Weinstein, D.L. et al. (1955) J. Bacteriol. 170:4223-30.

Cat. # Product Contents Size Price License Units Select
RR100 O-157 (Verocytotoxin Genes) PCR Screening Kit 200 Tests $572.00 License Statements



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