Advantage Ultrapure Nucleotides is the ideal dNTP mix to use for PCR, reverse transcription (RT) PCR, and real-time PCR. Each lot of Advantage dNTP mix is analyzed by chromatography, using Mono Q HR, to ensure a minimum of 99% triphosphates. Each lot is guaranteed to be free of nuclease activity.
Advantage Ultrapure dNTPs come either as a combination kit containing 100 mM of each dNTP in separate tubes or premixed with 10 mM per dNTP, both as aqueous solutions at pH 7.5. Under these conditions, the triphosphate forms of these nucleotides are very stable.