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Products >  PCR >  Molecular_Diagnostic_Products >  Titanium_Taq_SP_DNA_Polymerase

High-Yield DNA Polymerase for Laboratory Testing—Titanium Taq SP

Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP is a highly sensitive, robust, hot-start enzyme for use in PCR applications. It is ideal for PCR amplification of most DNA templates, in particular, complex genomic DNA. This product is manufactured under rigorous standards for quality, performance, and reliability to ensure that results are accurate and consistent from sample to sample.

Titanium Taq SP DNA Polymerase is a high-performance polymerase for commercial and OEM use, including molecular diagnostic services and kits. Commercial Use of Titanium Taq SP may provide cost-savings on royalty and license fees in some situations. If you are interested in Commercial Use of our products, including OEM, please contact Business Development at BD_OEM@clontech.com to discuss your needs.

The polymerase in all Titanium products is more robust than other Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5’-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive. This SP product performs similarly to standard Titanium Taq DNA Polymerase in all tested customer applications.

Titanium Taq is especially useful in demanding multiplex assays, where protocols are designed to efficiently assess more than one PCR target at a time, using multiple primer sets. This application requires high specificity to avoid mispriming events, as well as the ability to accommodate a variety of Mg2+ concentrations. Titanium Taq is a recombinant polymerase which has been carefully designed for enhanced solubility, resulting in increased sensitivity. Titanium Taq’s tolerance to a wide range of Mg2+ concentration enables more robust performance with targets of variable melting temperatures and amplification efficiencies.

Major Advantages Over Other DNA Polymerases

Titanium Taq allows you to:

  • Amplify your target using fewer PCR cycles, thus reducing background (see Images & Data tab)
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations (see Images & Data tab)
  • Amplify rare targets
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA. Targets of up to 4 kb can be readily amplified when the template is a moderately abundant cDNA or a less complex genomic DNA

Titanium Taq SP contains the same polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. This SP product performs similarly to standard Titanium Taq DNA Polymerase in all tested customer applications.

Integrated Hot Start for Higher Specificity

Titanium Taq comes preblended with TaqStart Antibody for higher specificity than other traditional Taq-based methods. This hot-start antibody, which is quickly inactivated at the onset of thermal cycling, blocks polymerase activity during the PCR setup, preventing the formation of primer dimers and other artifacts prior to thermal cycling. It is especially useful for multiplex PCR as it limits the number of mispriming events caused by multiple primer sets, which can result in spurious PCR products or smeared gel bands.

If you are purchasing this polymerase for research use only, please visit our standard Titanium Taq DNA Polymerase product page.

NOTICE TO PURCHASER: General Purpose Reagent. This product is intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this the product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact BD_OEM@clontech.com.

Your use of this product is also subject to compliance with the licensing requirements listed below and described on the product´s web page at http://www.clontech.com. It is your responsibility to review, understand and adhere to any restrictions imposed by these statements.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Manufactured and labeled in accordance with requirements for general purpose reagents
  • Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
  • Amplify your target using fewer PCR cycles, thus reducing background
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
  • Amplifies rare or low-copy targets
  • Set up your PCR at room temperatures and see increased specificity and yield due to the integrated hot-start antibody
  • Bring high performance to commercial and OEM use with cost-savings on royalty and license fees in some situations. (Contact Business Development at BD_OEM@clontech.com to discuss your needs.)

Applications

  • Genotyping
  • Whole genome PCR
  • Multiplex PCR
  • Primer extension
  • Hot-start PCR
  • Preparative PCR
  • Rare template amplification

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
639292 Titanium® Taq SP DNA Polymerase 100 Rxns $140.00
 

 

 
 

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