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Products >  PCR >  Molecular_Diagnostic_Products >  PrimeSTAR_GXL_SP_DNA_Polymerase

High-Fidelity DNA Polymerase for Laboratory Testing—PrimeSTAR GXL SP

PrimeSTAR GXL SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. PrimeSTAR GXL SP is a high-fidelity, robust, hot-start enzyme for use in PCR applications. It provides efficient PCR amplification even in challenging situations, such as when working with GC-rich templates, when excess template is present in the reaction, and when amplifying long products up to 30 kb. This product is manufactured under rigorous standards for quality, performance, and reliability to ensure that results are accurate and consistent from sample to sample.

PrimeSTAR GXL SP DNA Polymerase is a high-performance polymerase for commercial and OEM use, including molecular diagnostic services and kits. Commercial Use of PrimeSTAR GXL SP may provide cost-savings on royalty and license fees in some situations. If you are interested in Commercial Use of our products, including OEM, please contact Business Development at BD_OEM@clontech.com to discuss your needs.

The polymerase in all PrimeSTAR GXL products is a modified version of the PrimeSTAR HS enzyme that includes an elongation factor, thus enabling unsurpassed processivity. As the most robust high-fidelity polymerase available, PrimeSTAR GXL DNA Polymerase routinely allows amplification of PCR products that cannot be amplified by other enzymes. This polymerase saves time by minimizing optimization efforts and amplifying targets regardless of PCR conditions, and its antibody-mediated hot-start formulation enhances PCR specificity and sensitivity.

The strong processivity of this enzyme supports even coverage in next-generation sequencing (NGS). Robust amplification brings high performance to NGS experiments, especially when working with GC-rich targets and FFPE samples. These qualities, along with high specificity in library prep, lead to success in challenging NGS applications like long-range PCR in targeted resequencing.

This enzyme also displays outstanding performance in reactions containing excessive nucleic acids. Extraneous DNA ordinarily inhibits amplification by conventional polymerases because the amount of effective polymerase is limited by nonspecific binding. Thanks to the superior processivity of PrimeSTAR GXL DNA Polymerase, inhibition by excessive nucleic acids is limited, resulting in a much higher PCR success rate.

PrimeSTAR GXL SP contains the same polymerase as our standard PrimeSTAR GXL products, but with a modified, license-free buffer formulation. This SP product performs similarly to standard PrimeSTAR GXL DNA Polymerase in all tested customer applications.

If you are purchasing this polymerase for research use only, please visit our standard PrimeSTAR GXL DNA Polymerase product page.

NOTICE TO PURCHASER: General Purpose Reagent. This product is intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this the product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact BD_OEM@clontech.com.

Your use of this product is also subject to compliance with the licensing requirements listed below and described on the product´s web page at http://www.clontech.com. It is your responsibility to review, understand and adhere to any restrictions imposed by these statements.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Manufactured and labeled in accordance with requirements for general purpose reagents
  • Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
  • Highest processivity of all commercially-available high-fidelity DNA polymerases
  • Antibody-mediated hot-start formulation promotes successful, robust, and high-fidelity PCR regardless of conditions
  • Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA) or 13.5 kb (human cDNA)
  • Easy amplification of GC- or AT-rich templates and targets containing repeats
  • High tolerance for GC-rich templates, excess nucleic acids, and amplification of long products
  • Buffer and dNTPs are conveniently provided in the kit
  • Bring high performance to commercial and OEM use with cost-savings on royalty and license fees in some situations. (Contact Business Development at BD_OEM@clontech.com to discuss your needs.)

Applications

  • Amplification prior to NGS
  • PCR with GC-rich, AT-rich, or repetitive sequences
  • Cloning
  • Long-range PCR
  • Sequencing
  • Rare template amplification
  • PCR with limiting sample amounts

Components

RR220Q

PrimeSTAR GXL SP DNA Polymerase (1.25 U/µL)250 U
5X PrimeSTAR GXL SP Buffer2 x 1 ml
dNTP Mixture800 µl

Termini of PCR Products

PrimeSTAR GXL SP DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Storage

    -20°C. Avoid repeated freeze-thaw cycles.

Recommended Maximum Amplicon Length

  • Up to 30 kb (human genomic DNA template)
  • Up to 40 kb (lambda DNA template)
  • Up to 13.5 kb (human cDNA template)

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RF220Q PrimeSTAR® GXL SP DNA Polymerase 250 U $201.00 License Statements
 

 

 
 

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