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LA Taq DNA Polymerase, Hot-Start Version

TaKaRa LA Taq DNA Polymerase Hot-Start Version consists of Takara LA Taq polymerase plus a monoclonal antibody specific to Taq polymerase. This thermostable enzyme retains all of the high performance features of Takara LA Taq polymerase while increasing reaction specificity and reducing background. Takara LA Taq DNA Polymerase is optimized for long range PCR, enabling synthesis of products up to 30 kb for genomic DNA and 48 kb for lambda DNA templates. The polymerase also has 6.5X higher fidelity than Taq DNA Polymerase. The use of LA Taq polymerase requires little optimization and provides greater product yield compared to other manufacturers' long range PCR enzymes due to a robust enzyme-buffer system. Hot start PCR amplification reactions can be assembled at room temperature using TaKaRa LA Taq DNA Polymerase Hot-Start Version.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing,or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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Features

  • 6.5X higher fidelity amplifications compared with conventional Taq DNA Polymerase.
  • Less optimization and greater yield due to a robust enzyme-buffer system.
  • Reduced background and increased reaction specificity.

Applications

  • Long range PCR to amplify products up to 48 kb
  • Multiplex PCR
  • Amplification prior to sequencing with next-generation sequencing (NGS) platforms

Components

RR042A

Takara LA Taq DNA Polymerase Hot Start Version 125 U (5 U/µL)*
10X LA PCR Buffer II (Mg2+)25 mM
dNTP Mixture (2.5 mM each dNTP)400 µL
*Protocol recommends the use of 2.5 U per 50 µL reaction.

Storage

–20°C. Avoid repeated freeze-thaw of Takara LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Products

PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.

PCR Performance

Performance confirmed by successful amplification of a 35 kb lambda DNA template and a 17.5 kb human genomic DNA. Inhibition of Takara LA Taq activity by the antibody is confirmed to be >90% following enzyme incubation at 55°C for 10 min.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR042A TaKaRa LA Taq® DNA Polymerase Hot-Start Version 125 Units $181.00 License Statements
RR042B TaKaRa LA Taq® DNA Polymerase Hot-Start Version 500 Units $676.00 License Statements
 

 

 
 

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