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Products >  PCR >  Long_PCR >  LA_PCR_Kit

LA PCR Kit

The Takara-brand LA PCR Kit, Version 2.1 includes all the reagents needed for amplification of long DNA templates. With this kit, amplification of products up to 30 kb in length is routine. For some DNA templates, amplification of up to 48 kb is possible during long PCR. This long range PCR kit combines the Takara LA Taq enzyme with an optimized buffer system, resulting in longer and more accurate PCR products compared with conventional PCR kits. High fidelity PCR (6.5-fold higher than conventional Taq DNA Polymerase) is facilitated by the efficient 3' to 5' exonuclease activity of LA Taq enzyme. The Long Range PCR Kit contains a control template and primers to ensure optimal PCR performance during long PCR.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Genomic DNA template amplification up to 30 kb
  • Lambda DNA template amplification up to 48 kb
  • Longer and more accurate PCR products
  • Specialized 2X GC buffer provided for high-GC content or extensive secondary structure templates

Components

RR013A

Takara LA Taq 125 U (5 U/µL)
10X LA PCR Buffer II (with 25 mM Mg2+) 250 µL
10X LA PCR Buffer II (without Mg2+)250 µL
MgCl2 (25 mM) 500 µL
dNTP Mixture (2.5 mM each dNTP)400 µL
Control Template (100 ng/µL HT29 DNA)10 µL
Control Primer LA3 (10 pmol/µL)* 10 µL
Control Primer LA4 (10 pmol/µL)*10 µL
Lambda-Hind III MW Markers (100 ng/µL)20 µL
2X GC Buffer I (contains 5 mM MgCl2) 1.25 mL
2X GC Buffer II (contains 5 mM MgCl2) 1.25 mL
Control Primer GC1 (10 pmol/µL)** 10 µL
Control Primer GC2 (10 pmol/µL)** 10 µL

* Amplifies a 17.5 kb region of the control template.
** Amplifies a 1,255 bp GC-rich region of the control template.

Storage

–20°C

PCR Properties

PCR products generated with Takara LA Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR013A LA PCR™ Kit, Version 2.1 50 Rxns $241.00 License Statements
RR013B LA PCR™ Kit, Version 2.1 100 Rxns $464.00 License Statements
 

 

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