Affymetrix recommends Titanium DNA Amplification Kits for use with its CytoScan HD and CytoScan 750K* Arrays, as well as the Genome-Wide Human SNP Array 6.0 and the original GeneChip Human Mapping 250K and 500K Array Sets. These state-of-the-art SNP genotyping systems use Affymetrix’s proprietary GeneChip technologies to interrogate as many as 906,600 SNPs (in the SNP Array 6.0) or 750,000 SNPs (in the CytoScan HD Array). The CytoScan HD Array is relied upon for high-resolution cytogenetic analysis, allowing detection of human chromosomal aberrations with >99% sensitivity. CytoScan HD permits identification of 25–50 kb copy number changes across the genome at high specificity with SNP (allelic) call corroboration, covering all OMIM and RefSeq genes. Affymetrix evaluated DNA polymerases from several manufacturers and decided to exclusively recommend Titanium Taq DNA Polymerase for genome amplification on the basis of its superior performance and quality.
The Titanium DNA Amplification Kits were designed in collaboration with Affymetrix scientists and come in two formats. Cat. No. 639240 contains enough reagents to perform 300 reactions, while Cat. No. 639243, recommended for use with CytoScan HD, contains sufficient reagent for 400 reactions. The 300 reaction kit can be used by itself to support the original two-chip format, enabling you to screen 96 genomic DNA samples with the Mapping 500K Array Set. The 400 reaction kit can be used to support the single chip format, enabling you to screen 48 genomic DNA samples with the SNP Array 6.0. Use Titanium DNA Amplification Kits in conjunction with these powerful Affymetrix SNP Array Technologies to maximize the yield and quality of your SNP genotyping and whole-genome array studies.
Robust PCR Polymerase
Titanium DNA Amplification Kits feature Titanium Taq DNA Polymerase, a 5'-exonuclease deficient Taq polymerase blended with TaqStart Antibody for automatic hot start PCR. The kits also contain an optimized buffer mix (containing MgCl2), purified dNTP mix, and our proprietary RNase-free GC-Melt Reagent, which improves PCR amplification and yield when using templates with high GC-content or complex secondary structure.
Affymetrix GeneChip Technology
All of the Affymetrix GeneChip and Genome-Wide arrays provide high coverage across different populations. The CytoScan HD Array features over 2.6 million copy number markers, including 750,000 SNPs allowing detection of breakpoints, loss of heterozygosity (LOH) determination, regions identical-by-descent, maternal contamination, and low-level mosaicism (1). The SNP Array 6.0 features more than 1.8 million markers for genetic variation—including more than 906,600 SNPs and more than 946,000 probes for the detection of copy number variation—allowing high-performance, high-powered, low-cost genotyping. The Mapping 500K Array Set is comprised of two arrays, each capable of genotyping an average of 250,000 SNPs (2).
PCR Performance and Quality
Titanium DNA Amplification Kits meet our high-quality standards and have been functionally validated by Affymetrix scientists for use with their CytoScan HD, CytoScan 750K*, and SNP Array 6.0, as well as the original Mapping 250K and 500K Arrays. This extra validation step ensures that the Titanium amplification step will never be a roadblock to your genotyping and cytogenetics success.
*CytoScan 750K Arrays are not available from Affymetrix in the US or Canada.