Titanium Taq DNA Polymerase lacks the 5'-exonuclease activity of wild-type Taq, which makes it more robust than other Taq polymerases and allows it to amplify highly complex DNA mixes. This novel enzyme also contains carefully engineered amino acid substitutions that increase its solubility, making it the most sensitive PCR polymerase available (2).
Major Advantages Over Other DNA Polymerases
Titanium Taq allows you to:
- Amplify your target using fewer PCR cycles while reducing background (see Images & Data tab)
- Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of magnesium concentrations (see Images & Data tab)
- Amplify rare targets
- Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA. Targets of up to 4 kb can be readily amplified when the template is a moderately abundant cDNA or a less complex genomic DNA
Integrated Hot Start for Higher Specificity
Titanium Taq comes preblended with TaqStart Antibody. This hot start antibody, which is quickly inactivated at the onset of thermal cycling, blocks polymerase activity during the PCR reaction set-up, preventing the formation of primer-dimers and other artifacts prior to thermal cycling. This integrated hot start feature allows for higher specificity than other traditional Taq-based methods. It is especially useful for multiplex PCR because it limits the number of mispriming events caused by multiple primer sets, which can result in spurious PCR products or smeared gel bands.
A ready-to-use, lyophilized PCR master mix featuring Titanium Taq DNA Polymerase is also available as High Yield PCR EcoDry Premix. A Special Polymerase (SP) version is also available in liquid and lyophilized formats, for OEM and commercial use.
If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at email@example.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.