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Products >  PCR >  High_Yield_PCR >  Titanium_Taq_DNA_Polymerase

DNA Polymerase & PCR Kits—Titanium Taq

Titanium Taq DNA Polymerase is a highly sensitive, robust enzyme for use in all PCR applications. It’s ideal for the PCR amplification of any DNA template, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. Titanium Taq includes TaqStart Antibody, which provides an integrated hot start for increased specificity and yield (1).

Titanium Taq DNA Polymerase lacks the 5'-exonuclease activity of wild-type Taq, which makes it more robust than other Taq polymerases and allows it to amplify highly complex DNA mixes. This novel enzyme also contains carefully engineered amino acid substitutions that increase its solubility, making it the most sensitive PCR polymerase available (2).

Major Advantages Over Other DNA Polymerases

Titanium Taq allows you to:

  • Amplify your target using fewer PCR cycles while reducing background (see Images & Data tab)
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of magnesium concentrations (see Images & Data tab)
  • Amplify rare targets
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA. Targets of up to 4 kb can be readily amplified when the template is a moderately abundant cDNA or a less complex genomic DNA

Integrated Hot Start for Higher Specificity

Titanium Taq comes preblended with TaqStart Antibody. This hot start antibody, which is quickly inactivated at the onset of thermal cycling, blocks polymerase activity during the PCR reaction set-up, preventing the formation of primer-dimers and other artifacts prior to thermal cycling. This integrated hot start feature allows for higher specificity than other traditional Taq-based methods. It is especially useful for multiplex PCR because it limits the number of mispriming events caused by multiple primer sets, which can result in spurious PCR products or smeared gel bands.

A ready-to-use, lyophilized PCR master mix featuring Titanium Taq DNA Polymerase is also available as High Yield PCR EcoDry Premix. A Special Polymerase (SP) version is also available in liquid and lyophilized formats, for OEM and commercial use.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Amplify your target using fewer PCR cycles while reducing background
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of magnesium concentrations
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
  • Amplifies rare or low copy targets
  • Integrated hot start Ab allows room temperature reaction set-up and increased specificity
  • Available in a lyophilized format as High Yield PCR EcoDry Premix, which is ideal for room temperature storage and convenience

Applications

  • Genotyping
  • Whole Genome PCR
  • Multiplex PCR
  • Primer extension
  • Hot start PCR
  • Preparative PCR
  • Rare template amplification

References

  1. Kellogg, D. E. et al. (1994) BioTechniques 16(6):1137–1137.
  2. Barnes, W. M. (1992) Gene 112(1):29–35.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
639276 High Yield PCR EcoDry™ Premix 48 Rxns $84.00 License Statements
639278 High Yield PCR EcoDry™ Premix 24 Rxns $48.00 License Statements
639208 Titanium® Taq DNA Polymerase 100 Rxns $143.00 License Statements
639209 Titanium® Taq DNA Polymerase 500 Rxns $598.00 License Statements
639242 Titanium® Taq DNA Polymerase 1,000 Rxns $1,066.00 License Statements
639210 Titanium® Taq PCR Kit 100 Rxns $273.00 License Statements
639211 Titanium® Taq PCR Kit 30 Rxns $132.00 License Statements
639292 Titanium® Taq SP DNA Polymerase 100 Rxns $143.00  
 

 

 
 

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