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Products >  PCR >  High_Yield_PCR >  Ex_Taq_Premix

Premix Ex Taq

Takara-brand Premix Ex Taq is an optimized 2X PCR master mix composed of Takara Ex Taq enzyme, Ex Taq reaction buffer (Mg2+), and dNTP mixture. Premix Ex Taq is designed for quick and easy assembly (via dilution only) of the PCR master mix.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • High yield, high sensitivity PCR
  • Premixed PCR master mix for convenient amplification requiring minimal assembly
  • Generation of PCR products having >80% cloning efficiency in T-vectors

Components

RR003A

Takara Premix Ex Taq* 6 x 500 µL

*Contains Takara Ex Taq, Ex Taq Buffer, 4 mM MgCl and dNTPs.

Storage

–20°C. Avoid repeated freeze-thaw and vigorous stirring of product. Once thawed, aliquot product into PCR tubes and store at –20°C.

PCR Performance

Enzyme performance is confirmed by successful amplification of a 20 kb lambda DNA template. PCR amplification of a single copy gene (Beta-globin) by PCR is confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb).

PCR Products

PCR products generated with Ex Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors.

References

  1. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA 91:2216-2220.
  2. Cheng, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5695-5699.
  3. Cheng, S. et al. (1994) Nature Genet. 7:350-351.
  4. Cheng, S. et al. (1994) Nature 369:684-685.
  5. Saiki, R.K. et al. (1988) Science 239:487-491.
  6. Scharf, S.J. et al. (1986) Science 233:1076-1078.
  7. Gyllensten, U.B. & Erlich, H.A. (1988) Proc. Natl. Acad. Sci. USA 85:7652-7656.
  8. Kawasaki, E.S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:5698-5702.
  9. Lawyer, F.C. et al. (1988) J. Biol. Chem. 264:6427-6437.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR003A Premix Taq™ DNA Polymerase (Ex Taq™ Version 2.0) 120 Rxns $161.00 License Statements
 

 

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