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Products >  PCR >  High_Yield_PCR >  Ex_Taq_DNA_Polymerase_Hot-Start

Ex Taq DNA Polymerase, Hot-Start Version

Takara Ex Taq HS DNA Polymerase offers the same high performance as the standard Takara Ex Taq polymerase including high yield, excellent sensitivity, and fidelity 4.5X better than Taq Polymerase, along with the advantages of hot-start: lower background, increased specificity, and room temperature reaction assembly.

Takara Ex Taq HS DNA Polymerase includes a monoclonal antibody to Taq DNA Polymerase that binds to the polymerase and prevents nonspecific amplification due to mispriming and/or formation of primer dimers during reaction assembly. The antibody is then denatured during the initial PCR DNA-denaturation step, releasing the polymerase and allowing DNA synthesis to proceed.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • High yield and high sensitivity PCR
  • Excellent on difficult templates
  • Robust polymerase
  • Antibody mediated hot-start: lower background, increased specificity, and room temperature reaction assembly

Applications

  • Robust, specific amplification with reduced background
  • Amplifications requiring room-temperature reaction assembly

Components

RR006A

Takara Ex Taq HS 250 U (5 U/µL)*
10X Ex Taq Buffer (contains 20 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL

*Protocol recommends use of 1.25 U per 50 µL reaction.

RR030A Premix Ex Taq Hot Start Version

Takara Ex Taq HS, Premix**5 x 500µL

**Contains Takara Ex Taq HS, 1.25 units/25 µL; dNTP Mixture, 2X conc.; ea. 0.4 mM Ex Taq Buffer, 2X conc.; including 4 nM Mg2+.

Storage

–20°C. Avoid repeated freeze-thaws and vigorous stirring. Once thawed, aliquot into tubes and store at –20°C.

PCR Products

PCR products generated with Takara Ex Taq HS contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency in T-vectors.

PCR Performance

Enzyme performance is confirmed by successful amplification of a 20 kb lambda DNA template. Amplification of a single copy gene (beta-globin) by PCR is also confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb). Inhibition of Takara Ex Taq activity by the antibody is confirmed to be more than 90% after an incubation at 55°C for 10 min.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR030A Premix Ex Taq™ DNA Polymerase Hot Start Version 100 Rxns $164.00 License Statements
RR006A TaKaRa Ex Taq® DNA Polymerase Hot-Start Version 250 Units $207.00 License Statements
RR006B TaKaRa Ex Taq® DNA Polymerase Hot-Start Version 1,000 Units $736.00 License Statements
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $58.00
9002A Taq Antibody 250 Units $114.00
 
 
 

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