- High yield and high sensitivity PCR
- Excellent on difficult templates
- Robust polymerase
- Antibody mediated hot-start: lower background, increased specificity, and room temperature reaction assembly
- Robust, specific amplification with reduced background
- Amplifications requiring room-temperature reaction assembly
|Takara Ex Taq HS ||250 U (5 U/µL)*|
|10X Ex Taq Buffer (contains 20 mM MgCl2)||1 mL|
|dNTP Mixture (2.5 mM each dNTP)||800 µL|
*Protocol recommends use of 1.25 U per 50 µL reaction.
RR030A Premix Ex Taq Hot Start Version
|Takara Ex Taq HS, Premix**||5 x 500µL|
**Contains Takara Ex Taq HS, 1.25 units/25 µL; dNTP Mixture, 2X conc.; ea. 0.4 mM Ex Taq Buffer, 2X conc.; including 4 nM Mg2+.
–20°C. Avoid repeated freeze-thaws and vigorous stirring. Once thawed, aliquot into tubes and store at –20°C.
PCR products generated with Takara Ex Taq HS contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency in T-vectors.
Enzyme performance is confirmed by successful amplification of a 20 kb lambda DNA template. Amplification of a single copy gene (beta-globin) by PCR is also confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb). Inhibition of Takara Ex Taq activity by the antibody is confirmed to be more than 90% after an incubation at 55°C for 10 min.
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.