EmeraldAmp Max HS PCR Master Mix from Takara Bio includes a high yield, Hot Start (HS) PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (green) and a density reagent in a 2X Taq PCR Master Mix format. As a result, only primers and DNA template need to be added to initiate the reaction. This Taq PCR Master Mix can be used for routine PCR applications and completed reactions can be analyzed directly via gel electrophoresis. EmeraldAmp Max HS PCR Master Mix easily amplifies targets up to ~10 kb (genomic DNA) and works well with AT- or GC-rich templates. This Taq PCR Master Mix can also yield at least 10X more product than conventional PCR and exhibits improved performance over standard Taq polymerase.
The EmeraldAmp Max HS PCR Master Mix offers a powerful, flexible and convenient option for high yield, simple PCR applications.
If 5 μL of the reaction mixture is used for electrophoresis with 1% Agarose L03, the blue dye marker is detected near 3~5 kb and the yellow dye marker is detected below 50 bp. These dyes exhibit absorbance at 260nm and 420nm, respectively; it is recommended that they be removed from the gel prior to conducting any downstream fluorescent applications. Dyes can be removed by direct cutting out of the gel or performing DNA extractions.