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Products >  PCR >  General_Purpose >  Taq_and_Premix_Taq

TaKaRa Taq DNA Polymerase and Premix Taq DNA Polymerase

TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs. This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.

TaKaRa Taq DNA Polymerase is a recombinant and thermostable 94 kDa Taq polymerase encoded by a DNA polymerase gene derived from the Thermus aquaticus YT-1 strain and cloned into Escherichia coli. TaKaRa Taq DNA Polymerase has been demonstrated to have the same characteristics and capabilities as native Taq DNA polymerase.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Excellent for standard PCR amplifications
  • Low bacterial DNA contamination enzyme
  • Premix Taq enables easy PCR setup and minimizes pipetting steps

Applications

  • Routine PCR
  • DNA sequencing

Components

R001A

Takara Taq250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 

R001AM

Takara Taq250 U (5 U/µL)*
10X PCR Buffer (without Mg2+)1 mL
25 mM MgCl2 1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 

R004A - Premix Taq (Takara Taq Version)

2X Taq Polymerase Premix 6 x 500 µL**

*Protocol recommends the use of 1.25 U per 50 µL reaction.
**Contains Takara Taq, PCR Buffer and dNTPs.

Source

Recombinant E. coli

Storage

–20°C. Avoid repeated freeze-thaw of Takara Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Performance

Enzymatic performance is confirmed by successful amplification of an 8 kb lambda DNA template. PCR amplification of a single copy gene is also confirmed using a 2.9 kb human genomic DNA template and human p53 primers.


 
 
Products
Cat. # Product Contents Size Price License Units Select
R004A Premix Taq™ DNA Polymerase (TaKaRa Taq™ Version 2.0) 150 Units $134.00 License Statements
R001A TaKaRa Taq™ DNA Polymerase 250 Units $124.00 License Statements
R001B TaKaRa Taq™ DNA Polymerase 1,000 Units $432.00 License Statements
R001C TaKaRa Taq™ DNA Polymerase 3,000 Units $1,137.00 License Statements
R001AM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 250 Units $124.00 License Statements
R001CM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 3,000 Units $1,137.00 License Statements
R001BM TaKaRa Taq™ DNA Polymerase (with Mg2+ free buffer) 1,000 Units $432.00 License Statements
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $58.00
 
 
 

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