Features- Amplification of DNA from any template via PCR
Components R011 | | Takara Taq (5 U/µL) | 250 U | | dNTP Mixture (2.5 mM each) | 1.28 mL | | 10X PCR Buffer (contains 15 mM MgCl2) | 1 mL | | 10X PCR Buffer (without Mg2+) | 1 mL | | MgCl2 (25 mM) | 1 mL | | Control Template (1 µg/mL lambda DNA) | 100 µL | | Control Primer 1 (20 pmol/µL) | 50 µL | | Control Primer 2 (20 pmol/µL) | 50 µL | | Control Primer 3 (20 pmol/µL) | 50 µL | | Lambda EcoT 14 I Marker (100 ng/µL) | 40 µL | | 6X Loading Buffer | 1 mL |
(36% glycerol, 0.05% Bromophenol blue, 30mM EDTA, 0.05% xylene cyanol)
Storage –20°C. Avoid repeated freeze-thaw of Takara Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.
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