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Products >  PCR >  General_Purpose >  Hot_Start_Taq

Hot Start PCR Enzyme: Hot Start Takara Taq DNA Polymerase

These hot start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody to Taq polymerase. The antibody binds Taq polymerase, thereby preventing DNA synthesis. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot start PCR enzyme prevents nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly.

Several hot start versions of Takara Taq are available:

TaKaRa Taq DNA Polymerase Hot Start Version: enzyme, buffer, and dNTPs are supplied as separate components.

Premix Taq DNA Polymerase Hot Start Version: 2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer.

TaKaRa Taq HS Perfect Mix: 2X premix optimized for fast reactions; compatible with a 20 sec./kb extension step.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • All hot start Takara Taq versions contain an anti-Taq polymerase monoclonal antibody that improves specificity, reduces background, and allows room temperature assembly
  • TaKaRa Taq DNA Polymerase Hot Start Version: enzyme, buffer, and dNTPs are supplied as separate components.
  • Premix Taq DNA Polymerase Hot Start Version: 2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer.
  • TaKaRa Taq HS Perfect Mix: 2X premix optimized for fast reactions; compatible with a 20 sec./kb extension step.

Applications

  • Hot start PCR
  • Multiplex PCR

Components

TaKaRa Taq DNA Polymerase, Hot Start Version (Cat. #R007A) 

Takara Taq  250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2)1 mL
dNTP Mixture (2.5 mM each dNTP)800 µL
 * Use 1.25 U per 50 µL reaction.

Premix Taq DNA Polymerase, Hot Start Version (Cat. # R028A) 

2X Takara Taq, Premix*5 X 500 µL
 * 2X dNTP Mixture (0.4 mM each) and 2X PCR buffer (20 mM Tris-HCl (pH 8.3), 100 mM KCI, and 3 mM MgCl2)

TaKaRa Taq Perfect Mix (Cat. # R300A)

 
Takara Taq HS Perfect Mix (2X) 5 X 500 µL    

Storage

–20°C. Avoid repeated freezing and thawing.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
R028A Premix Taq™ DNA Polymerase Hot-Start Version 100 Rxns $131.00 License Statements
R007A TaKaRa Taq™ DNA Polymerase Hot Start Version 250 Units $141.00 License Statements
R007B TaKaRa Taq™ DNA Polymerase Hot Start Version 1,000 Units $512.00 License Statements
R300A TaKaRa Taq™ HS Perfect Mix 100 Rxns $101.00  
R300B TaKaRa Taq™ HS Perfect Mix 400 Rxns $408.00  
 

 

 
 

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