Taq Full DNA Polymerase is an ultrapure, highly efficient, full-length, recombinant version of the Thermus aquaticus (Taq) YT1 DNA Polymerase (94 kD; 1). As a thermostable DNA polymerase, it can withstand prolonged incubations at temperatures up to 95°C without significant loss of activity. The enzyme possesses two important catalytic activities: a 5'–3' polymerase activity, and a double-strand specific 5'–3' exonuclease activity. Like other full-length Taq DNA Polymerases, this enzyme lacks 3'–5' exonuclease (proofreading) activity.
Superior Sensitivity and Cost-Effective Performance
With Taq Full DNA polymerase you can efficiently and conveniently amplify DNA from any template. Therefore, this enzyme is recommended for any general PCR amplification procedure. Taq Full can generate higher yields than any other full-length Taq polymerase. In addition, it is able to amplify even rare targets from bacterial and plasmid DNA, cDNA, and complex genomic DNA. Its high efficiency and sensitivity allow you to use less enzyme and template in each reaction—letting you get the most mileage from each PCR reaction. Taq Full is also available with a hot start antibody (Taq Full Hot Start DNA Polymerase), providing heightened performance and guaranteeing increased specificity and yield.
No Optimization Required
Our Taq Full and Taq Full Hot Start DNA Polymerases allow you to perform PCR without tedious buffer optimization. In any given reaction, Taq Full tolerates a wide range of Mg2+ concentrations. To make the most of this feature, we have included MgCl2 at a set concentration in the Taq Full PCR Buffer, eliminating the need to optimize or add Mg2+ as a separate component during reaction setup. In our experiments, Taq Full was also shown to perform equally well in PCR buffers containing higher MgCl2 concentrations (Images & Data tab). The wide Mg2+ tolerance exhibited by this enzyme eliminates the need to optimize the Mg2+ concentration in your PCR assays, and saves considerable time and effort.
Hot Start Antibody for Specificity
Taq Full Hot Start DNA Polymerase contains an integrated hot start antibody for automatic hot start. Clontech’s hot start system blocks polymerase activity during the PCR reaction set-up, thus preventing the formation of primer-dimers and other artifacts of low-level synthesis prior to thermal cycling (2). The antibody is quickly inactivated at the onset of thermal cycling, allowing PCR to proceed as usual. Clontech’s hot start system, therefore, allows for higher specificity and promotes the best qualities of Taq Full DNA Polymerase.