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Products >  PCR >  GMP-Grade_Products >  TaKaRa_Taq_Hot_Start_Version_GMP_grade

GMP-Grade DNA Polymerase: Hot Start Takara Taq

TaKaRa Taq Hot Start Version GMP grade DNA polymerase is manufactured under rigorous standards to ensure high quality and consistency, in a facility compliant with GMP regulations and guidelines. These manufacturing practices bring an added level of confidence and consistency to a hot-start polymerase especially suited for demanding research applications, including multiplex PCR. This GMP-grade reagent offers:

  • Stringent specifications
  • Outstanding performance
  • Custom sizes and packaging

All hot-start Takara Taq polymerases contain a neutralizing anti-Taq monoclonal antibody that improves specificity, reduces background, and allows assembly at room temperature. This feature prevents nonspecific amplification and is therefore ideal for multiplex PCR, where high specificity is key to experimental success.The antibody is denatured during the initial denaturation step, allowing this product to be used with standard PCR conditions.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration,is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

  At-A-Glance   Documents   Images & Data

Features

Test Items
Specifications
Results
Purity (microfluidic chip analysis)>98%Passed
RibonucleasesNo observable degradationPassed
EndonucleasesNo observable degradationPassed
ExonucleasesNo observable degradationPassed
Nicking activitiesNo observable relaxation of supercoiled DNAPassed
Residual E. coli gDNA<10 copies/1.25 UPassed
Hot-start activityConfirmedPassed
PCR performance8-kb fragment was amplifiedPassed
Sensitivity (λDNA)0.5-kb fragment was amplified from 10 pg of λDNAPassed
Sensitivity (human gDNA)0.1-kb fragment was amplified from 30 pg of human genomic DNAPassed

Applications

  • Hot-start PCR
  • Multiplex PCR
  • qPCR

Components

TaKaRa Taq Hot Start Version GMP grade250 U (5 U/µl)
10X PCR Buffer (Mg2+ plus) GMP grade1 ml
10X PCR Buffer (Mg2+ free) GMP grade1 ml
25 mM MgCl2 GMP grade1 ml

Storage

–20°C. Avoid repeated freezing and thawing.

Additional Information

Please see the product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
R310A TaKaRa Taq™ Hot Start Version GMP grade 250 U $140.00 License Statements
 

 

 
 

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