- Robust enzyme-buffer system for difficult templates
- Optimized buffers (GC Buffers I & II) for amplification of templates with high GC content and/or significant secondary structure
- High-yield amplification of long DNA targets up to 48 kb is possible with minimal optimization
- Long and accurate PCR amplification of genomic DNA targets (long range PCR)
|Takara LA Taq ||125 U (5 U/µL)*|
|2X GC Buffer I (contains 5 mM MgCl2) ||1.25 mL|
|2X GC Buffer II (contains 5 mM MgCl2) ||1.25 mL|
|dNTP Mixture (2.5 mM each dNTP) ||400 µL|
|*Protocol recommends the use of 2.5 U per 50 µL reaction.|
–20°C. Avoid repeatedly freezing and thawing Takara LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.
PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
Performance is confirmed by successful amplification of a 35 kb lambda DNA template, and a 17.5 kb human genomic DNA with GC Buffer I. Amplification of the c-jun proto-oncogene (1,255 bp, 65% GC content) by PCR is also confirmed using human genomic DNA as the template with GC Buffer I and II.
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.