The Advantage Genomic LA Polymerase Mix, which is designed for long PCR, is an enzyme blend composed of full-length, thermostable Taq DNA Polymerase, a small amount of proofreading enzyme, and a hot start antibody. The 3' to 5' proofreading exonuclease activity removes misincorporated bases, allowing subsequent product extension to proceed quickly and efficiently, making amplification of long DNA fragments possible. It enables synthesis of PCR products up to 30 kb using human genomic DNA templates (20 kb from GC-rich human genomic DNA), and up to 48 kb using non-complex templates.
Premium Blend for Long & Accurate PCR
The key to length and accuracy is the blending of the thermostable DNA polymerase and the 3' to 5' exonuclease activity (proofreading enzyme). This premium blend makes Advantage Genomic LA Polymerase the best enzyme mix for long-distance PCR amplification.
The Advantage Genomic LA enzyme blend is suitable for hot start PCR due to the presence of a neutralizing monoclonal antibody that binds and inhibits the DNA polymerase at ambient temperatures. Nonspecific amplification, due to mispriming and/or formation of primer-dimers before thermal cycling, is prevented because the antibody inhibits polymerase activity by binding to the enzyme until the temperature rises.
Higher Fidelity & Increased Yield
The Advantage Genomic LA Polymerase enzyme blend provides 6.5X higher fidelity than wild-type Taq DNA Polymerase (internal data obtained as in reference 1) due to the proofreading enzyme’s efficient 3' to 5' exonuclease activity. This robust enzyme system also reduces background and provides greater product yields, with less optimization, than other "long and accurate (LA) polymerases". Additionally, the Advantage Genomic LA Polymerase Mix buffer has been optimized for high amplification yield and increased fidelity. PCR products generated with the enzyme mix contain a 3'-A overhang and are therefore suitable for cloning into TA-vectors.
Advantage GC Genomic LA Polymerase Mix for GC-Rich Templates
GC-rich templates have strong secondary structures that resist denaturation and prevent efficient primer annealing. The Advantage GC Genomic LA Polymerase Mix combines the benefits of the Advantage Genomic LA Polymerase enzyme blend, such as high efficiency and improved fidelity on long templates, with our Advantage GC-Melt buffer system, which weakens base pairing in GC-rich sequences. This allows efficient amplification of virtually all GC-rich sequences (up to 90% GC) that resist standard PCR amplification techniques. Improve your PCR yields with this highly effective mix for GC-rich templates.
These enzyme mixes display a high degree of sensitivity. Even on GC-rich targets, Advantage GC Genomic LA Polymerase is able to amplify regions of GC-rich DNA—such as a 1.9 kb region of the TGF-beta gene (GC content 69%)—from as little as 10 pg of template.