Advantage GC 2 Polymerase Mix is an excellent choice for GC rich PCR applications. For example, GC-rich templates, such as the 5' ends of many mammalian cDNAs, cannot be amplified using conventional PCR techniques. Advantage GC 2 includes Advantage 2 Polymerase Mix, our proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two proven additives allowing amplification of sequences that are up to 90% GC with minimal buffer optimization.
When used together, DMSO and GC-Melt reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques. The Advantage GC 2 Polymerase Mix and PCR Kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix (Cat. No. 639153).
If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or submit an inquiry.
