Advantage GC 2 Polymerase Mix is an excellent choice for GC rich PCR applications. For example, GC-rich templates, such as the 5' ends of many mammalian cDNAs, cannot be amplified using conventional PCR techniques. Advantage GC 2 includes Advantage 2 Polymerase Mix, our proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two proven additives allowing amplification of sequences that are up to 90% GC with minimal buffer optimization.
When used together, DMSO and GC-Melt reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques. The Advantage GC 2 Polymerase Mix and PCR Kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix (Cat. No. 639153).
If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at firstname.lastname@example.org to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.