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Products >  PCR >  Fast_PCR >  Z-Taq_DNA_Polymerase

Z-Taq DNA Polymerase

TaKaRa Z-Taq DNA Polymerase has greater processivity than standard Taq DNA polymerase and allows rapid PCR amplification. Using Z-Taq polymerase, a 1 kb DNA fragment can be amplified from genomic DNA in 20 minutes total (using a 30-cycle, two-step amplification protocol). The improved processivity of Z-Taq enzyme also enables the amplification of longer fragments than standard Taq DNA polymerase; human genomic DNA templates up to 17.5 kb, and lambda DNA templates of 20 kb have been successfully amplified.

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Features

  • Reaction speed 5 times faster than standard Taq DNA polymerase

Applications

  • Rapid PCR amplification

Components

R006A

Takara Z-Taq 200 U (2.5 U/µL)*
10X Z-Taq Buffer (with 30 mM MgCl2)800 µL
dNTP Mixture (2.5 mM each dNTP)800 µL
*1.25 U per 50 µL reaction is recommended

Storage

–20°C. Avoid repeated freezing and thawing of Takara Z-Taq enzyme and dNTPs. Once thawed, aliquot each component and store at –20°C.

PCR Products

PCR products generated with Takara Z-Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency when using T-vectors.

PCR Performance

Performance is assessed by rapid PCR amplification of a 10 kb lambda DNA target.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
R006A TaKaRa Z-Taq™ DNA Polymerase 200 Units $291.00 License Statements
R006B TaKaRa Z-Taq™ DNA Polymerase 800 Units $1,023.00 License Statements
 

 

 
 

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