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SpeedSTAR DNA Polymerase

SpeedSTAR HS DNA Polymerase is optimized for fast PCR. With an extension rate as fast as 10 sec/kb (compared to 60 sec/kb with standard Taq DNA polymerase), SpeedSTAR DNA polymerase dramatically reduces total reaction time. Fast PCR with SpeedSTAR can be performed using standard PCR machines, eliminating the requirement for special equipment.

With two optimized buffers, Fast Buffer I and II, SpeedSTAR DNA polymerase provides efficient amplification across a wide size range (up to 20 kb) with less optimization than other polymerases. In addition, the antibody-mediated hot-start formulation provides high specificity.

 

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@clontech.com to discuss your needs, or visit http://info.clontech.com/OEM/OEM_Inquiry.html to submit an inquiry.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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Features

  • Optimized for fast PCR: amplify a 2 kb fragment in as little as 30 minutes using a standard PCR machine
  • Excellent efficiency: robust performance, comparable to high yield polymerases
  • Optimized buffers allow amplification of fragments up to 20 kb

Applications

  • Fast PCR: reactions can be completed in two-thirds less time
  • Amplification of large DNA fragments

Components

RR070A

SpeedSTAR HS DNA Polymerase (5 units/µL)50 µL
10X Fast Buffer I (Mg2+*)1 mL
10X Fast Buffer II (Mg2+*)1 mL
dNTP Mixture (ea. 2.5 mM)800 µL

*Mg2+ Concentration: 10X Fast Buffer I, 30 mM; 10X Fast Buffer II, 20 mM

Purity

Nicking, endonuclease and exonuclease activities are not detected following incubation of 0.6 µg supercoiled pBR322, 0.6 µg lambda DNA or 0.6 µg lambda-Hind III digest with 10 units of SpeedSTAR DNA Polymerase for 1 hour at 74°C.

Storage

–20°C

PCR Products

The majority of PCR products generated with SpeedSTAR DNA Polymerase will have 3'-A overhangs; therefore, amplification products can be directly cloned into T-vectors. It is also possible to clone amplification products into blunt-ended vectors following blunting and phosphorylation of product ends.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR070A SpeedSTAR™ HS DNA Polymerase 250 Units $238.00 License Statements
RR070B SpeedSTAR™ HS DNA Polymerase 1,000 Units $866.00 License Statements
 

 

 
 

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