Direct PCR allows for amplification directly from the tissue source or crude extracts, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces cost. Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited.
How is it achieved?
Direct PCR relies on modified polymerases and buffer systems that enable DNA amplification in the presence of PCR inhibitors, growth serum, and other source material components.