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Products >  PCR >  Commercial-Use_Products >  Titanium_Taq_SP_DNA_Polymerase

High-Yield DNA Polymerase—Titanium Taq SP

Titanium Taq SP DNA Polymerase is a highly sensitive, robust, hot-start enzyme for use in PCR applications. It is outstanding for PCR amplification of most DNA templates, in particular, complex genomic DNA. A glycerol-free version (Cat. # 638517) is also available, ideal for preparation of custom lyophilized (dried-down) PCR master mixes that offer higher convenience and room-temperature stability. The EcoDry Premix version (Cat. # 638519) enables high performance with minimal effort and maximum ease, provided as a pre-aliquoted lyophilized master mix that already contains all the reagents needed for high-yield PCR in each tube.

Titanium Taq SP DNA Polymerase is specially formulated to provide a high-performance polymerase for commercial and OEM use, including molecular diagnostic services and kits. Commercial Use of Titanium Taq SP may provide cost-savings on royalty and license fees in some situations. If you are interested in Commercial Use of our products, including OEM, please contact Business Development at bd_oem@clontech.com to discuss your needs.

The polymerase in all Titanium products is more robust than other Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5’-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive. This SP product performs similarly to standard Titanium Taq DNA Polymerase in all tested customer applications.

Titanium Taq is especially useful in demanding multiplex assays, where protocols are designed to efficiently assess more than one PCR target at a time, using multiple primer sets. This application requires high specificity to avoid mispriming events, as well as the ability to accommodate a variety of Mg2+ concentrations. Titanium Taq is a recombinant polymerase which has been carefully designed for enhanced solubility, resulting in increased sensitivity. Titanium Taq’s tolerance to a wide range of Mg2+ concentration enables more robust performance with targets of variable melting temperatures and amplification efficiencies.

Major Advantages Over Other DNA Polymerases

Titanium Taq allows you to:

  • Amplify your target using fewer PCR cycles, thus reducing background (see Images & Data tab)
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations (see Images & Data tab)
  • Amplify rare targets
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA. Targets of up to 4 kb can be readily amplified when the template is a moderately abundant cDNA or a less complex genomic DNA

Titanium Taq SP contains the same polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. This SP product performs similarly to standard Titanium Taq DNA Polymerase in all tested customer applications.

Integrated Hot Start for Higher Specificity

Titanium Taq comes preblended with TaqStart Antibody for higher specificity than other traditional Taq-based methods. This hot start antibody, which is quickly inactivated at the onset of thermal cycling, blocks polymerase activity during the PCR setup, preventing the formation of primer dimers and other artifacts prior to thermal cycling. It is especially useful for multiplex PCR as it limits the number of mispriming events caused by multiple primer sets, which can result in spurious PCR products or smeared gel bands.

If you are purchasing this polymerase for research use only, please visit our Titanium Taq DNA Polymerase or Titanium Taq EcoDry Premix product page.

If you are purchasing this polymerase for use as a general purpose reagent (GPR), please visit our Laboratory Testing Titanium Taq SP DNA Polymerase product page.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Amplify your target using fewer PCR cycles, thus reducing background
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
  • Amplifies rare or low-copy targets
  • Set up your PCR at room temperatures and see increased specificity and yield due to the integrated hot-start antibody
  • Prepare your own lyophilized PCR master mixes with the glycerol-free version (Cat. # 638517)
  • Eco-friendly, lyophilized premix ideal for room temperature storage and convenience (Cat. # 638519)
  • Bring high performance to commercial and OEM use with cost-savings on royalty and license fees in some situations. (Contact Business Development at bd_oem@clontech.com to discuss your needs.)

Applications

  • Genotyping
  • Whole genome PCR
  • Multiplex PCR
  • Primer extension
  • Hot-start PCR
  • Preparative PCR
  • Rare template amplification
  • Custom master mix lyophilization (Cat. # 638517)

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
  639292 Titanium® Taq SP DNA Polymerase 100 Rxns Call *
  638517 50X Titanium® Taq SP (Glycerol-Free) 250 uL $350.00
638519 Titanium® Taq SP EcoDry™ Premix 48 Rxns $84.00
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 
 

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