NucleoSpin RNA XS allows the isolation of highly concentrated, highly pure total RNA from very dilute or low-volume tissue and cell samples without the use of organic compounds (e.g., Trizol).
New Column Design
The innovative funnel-shaped thrust ring allows standard miniprep spin columns to hold a silica membrane of very small diameter. The small surface of the NucleoSpin RNA XS membrane allows efficient elution in only 5–20 µl, resulting in the highest RNA concentrations. The purified RNA is ready for all typical downstream applications.
Procedure
Cells are lysed by incubation in the lysis buffer (high concentration of chaotropic salts). This lysis buffer immediately inactivates RNases—which are present in virtually all biological materials—and creates appropriate binding conditions which favor adsorption of RNA to the silica membrane. After lysis, homogenization and reduction of viscosity are achieved by filtration with NucleoSpin Filter units (shredders). Residual genomic DNA is efficiently removed by on-column digestion with rDNase, provided with the kit. Total RNA is eluted in RNase-free water. The low elution volume results in concentrated RNA with typical RIN (RNA Integrity Numbers) >9 (depending on sample quality).
