Features - Complete midi kit with rDNase for on-column digestion—includes shredders
- Purify total RNA from cells, tissue, and yeast
- Ready-to-use RNA for performance in any downstream applications
- No use of toxic material such as phenol/chloroform (e.g., Trizol), CsCl, or ethanol
Applications Isolate total RNA from cultured cells and tissue (standard protocol), as well as bacterial cells, yeast cells, and samples stored in RNAlater (support protocols). Purified RNA suitable for: - Real-time RT-PCR
- Northern blotting
- Primer extension
- Array technology
- RNase protection assays
Product Overview | Technology | Silica membrane | | Format | Midi spin columns | | Starting Material | < 5 x 107 cultured cells
< 1010 bacterial cells
200 mg tissue
< 3 x 108 yeast cells | | Fragment Size | 200 bp–20 kb | | Typical Yield | 180 µg from 107 HeLa cells
620 µg from 4 x 107 HeLa cells | | A260/280 | 1.90–2.10 | | Elution Volume | 500 µl | | Preparation Time | 80 min/4 preps | |
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