The NucleoTrap mRNA kit provides latex beads covalently modified with oligo(dT), which allow for isolation and purification of mRNA from total RNA or directly from cells or tissue.
Under high-salt conditions, poly(A) mRNA binds to the beads. The latex beads are then centrifuged, resuspended in washing buffer, and transferred to a NucleoSpin microfilter which simplfies handling of the beads. After two additional washing steps, poly(A) mRNA is eluted with nuclease-free water or low-salt buffer (since A-dT hybrids are destabilized at lower ionic strength). Using NucleoTrap mRNA ensures high yields of high-quality poly(A) mRNA, which is not degraded and is free of DNA contamination.
NucleoTrap mRNA kits contain spin filters, as well as all the necessary reagents and buffers for isolating highly pure poly(A) mRNA from total RNA preparations. The purified mRNA is ready for use in downstream applications, such as cDNA library construction, blotting techniques, RT-PCR, in vitro translation, expression arrays, and differential display. mRNA enrichment is especially recommended for construction of cDNA libraries and demanding blotting procedures, since it reduces background signals.