Purification of Viral RNA and DNA from Cell-Free Biological Fluids—NucleoSpin Virus
The NucleoSpin Virus kit is designed for efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently using a lysis buffer that consists of a highly concentrated chaotropic salt solution. DNA viruses, which are usually more difficult to lyse, also require a digestion with Proteinase K, which is supplied in the kit. Carrier RNA improves binding and recovery of dilute viral nucleic acids. A combination of the supplied lysis buffer and ethanol create appropriate conditions for nucleic acids to bind to the NucleoSpin Virus Columns. After three washing steps to remove salts, metabolites, and soluble macromolecular cellular components, the nucleic acids are eluted in water and are ready for use in downstream reactions.