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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Viral >  Single_Prep

Purification of Viral RNA and DNA from Cell-Free Biological Fluids—NucleoSpin Virus

The NucleoSpin Virus kit is designed for efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently using a lysis buffer that consists of a highly concentrated chaotropic salt solution. DNA viruses, which are usually more difficult to lyse, also require a digestion with Proteinase K, which is supplied in the kit. Carrier RNA improves binding and recovery of dilute viral nucleic acids. A combination of the supplied lysis buffer and ethanol create appropriate conditions for nucleic acids to bind to the NucleoSpin Virus Columns. After three washing steps to remove salts, metabolites, and soluble macromolecular cellular components, the nucleic acids are eluted in water and are ready for use in downstream reactions.

  At-A-Glance   Documents   Images & Data

Features

  • Suitable for cell-free body fluids, such as serum or plasma
  • Efficient recovery of viral nucleic acids from a 200 µl starting volume (or up to a 400 µl starting volume with two loading steps)
  • Nucleic acid isolation from human and animal samples
  • Proteinase K solution included for efficient sample lysis

Applications

  • Viral RNA and DNA purification from serum, plasma, and cell-free biological fluids
  • Typical downstream applications include RT-PCR and enzymatic reactions

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material <200 µl serum, plasma, cell-free biological fluids
(up to 400 µl with two loading steps)
Fragment Size 100 bp–approx. 50 kb
Elution Volume 30 µl
Preparation Time 50 min/6 preps
Binding Capacity 25 µg

 
 
Products
New Cat. # Product Contents Size Price Units Select
740983.10 NucleoSpin® Virus 10 Preps $45.00
740983.50 NucleoSpin® Virus 50 Preps $198.00
740983.250 NucleoSpin® Virus 250 Preps $867.00
 

 

 
 

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