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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Viral >  Multi_Prep

Isolation of Viral RNA or DNA—NucleoSpin 8/96 Virus & 8/96 Virus Core Kit

The NucleoSpin 8/96 Virus Kits are designed for the rapid and simultaneous preparation of viral RNA or viral DNA from serum, plasma, or any cell-free biological fluid. The kits allow reliable purification of viral RNA or DNA without cross-contamination. NucleoSpin 8 Virus and NucleoSpin 96 Virus are primarily designed for manual use in a centrifuge. NucleoSpin 8/96 Virus Core Kits allow for more variation and higher flexibility in the consumables used for lysis, washing, and elution; and are primarily recommended for manual or automated vacuum use.

With the NucleoSpin 8/96 Virus method, RNA viruses are lysed quickly and efficiently using a lysis buffer which is a highly concentrated solution of GITC. DNA viruses (e.g., HBV) are usually more difficult to isolate and require digestion of samples with proteinase K. The lysis buffer plus ethanol creates the appropriate conditions for binding of nucleic acids to the silica membrane of the NucleoSpin Virus Binding Strips or Plate. Carrier RNA included in lysis buffer improves the binding and recovery of low concentration viral RNA/DNA. Contaminants (potential PCR inhibitors) such as salts, metabolites, and soluble macromolecular cellular components are removed in washing steps with ethanolic wash buffers. The purified viral RNA or viral DNA can be eluted in low-salt buffer or water and is ready to use in downstream applications such as RT-PCR and PCR.

At-A-Glance Documents Images & Data Resources

Features

  • Isolate viral RNA/DNA from serum, plasma, or cell-free biological fluids
  • Wash plate is available to minimize risk of cross-contamination
  • Process under vacuum or by centrifugation
  • Suitable for manual and automated processing
  • Compatible with a variety of robotic systems

Applications

  • Isolate viral RNA/DNA from HCV, HBV, HAV, HIV, HPV, HSV, VZV, EBV, parvovirus B19, and H5N1.

Product Overview

NucleoSpin 8 Virus
NucleoSpin 8 Virus Core Kit

NucleoSpin 96 Virus
NucleoSpin 96 Virus Core Kit

Technology

Silica membrane

Silica membrane

Format

8-well strips

96-well plates

Processing

NucleoSpin 8 Virus
Manual, centrifugation
NucleoSpin 8 Virus Core Kit
Manual or automated,
vacuum or centrifugation

NucleoSpin 96 Virus
Manual, centrifugation
NucleoSpin 96 Virus Core Kit
Manual or automated,
vacuum or centrifugation

Starting Material

150 µl (100 cp/ml) serum, plasma, cell-free biological fluids

150 µl (100 cp/ml) serum, plasma, cell-free biological fluids

Fragment Size

100 bp–30 kb

100 bp–30 kb

Typical Recovery

>90%

>90%

Elution Volume

70–100 µl

70–100 µl

Preparation Time

60 min/6 strips

60 min/plate


 
 
Products
Cat. # Product Package Size Price # of Units Select
740643 NucleoSpin® 8 Virus 12 x 8 Preps $342.00
740643.5 NucleoSpin® 8 Virus 60 x 8 Preps $1,474.00
740451.4 NucleoSpin® 8 Virus Core Kit 48 x 8 Preps $853.00
740643.12S NucleoSpin® 8 Virus Strips 12 Strips $83.00
740691.2 NucleoSpin® 96 Virus 2 x 96 Preps $646.00
740691.4 NucleoSpin® 96 Virus 4 x 96 Preps $1,172.00
740452.4 NucleoSpin® 96 Virus Core Kit 4 x 96 Preps $853.00
 

 

 


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