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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Viral >  Magnetic_Pathogen

Magnetic Bead-Based Isolation of Viral RNA/DNA and Bacterial DNA—NucleoMag Pathogen

The NucleoMag Pathogen kit allows the purification of viral RNA and DNA and microbial DNA using magnetic beads. The kit is designed for common clinical samples, such as whole blood, serum or plasma, feces, tissue, or swabs. After a sample-specific pre-lysis procedure, 200 µl of liquid or homogenized sample is used in the purification procedure.

The NucleoMag product family is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA can be eluted from the beads using elution buffer.

The NucleoMag technology can easily be adapted to common liquid handling instruments. It can be used either with static pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

  At-A-Glance   Documents

Features

  • One kit for any common clinical sample type
  • High sensitivity
  • Reliable nucleic acid isolation—suitable even for low viral titers

Applications

  • Extraction of viral RNA and DNA and microbial DNA from clinical samples
  • Clinical testing applications

Product Overview

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads
ProcessingManual or automated
Starting materialThe prep is fully scalable. 
A convenient volume, especially for
96-well processing, would be:
<200 µl whole blood, serum, plasma
<30 mg tissue (e.g., ear notches)
<200 µl feces, <200 µl swab wash solution
Maximum amount of
starting material in
 purification procedure
200 µl liquid/homogenized sample
Fragment size 300 bp–approximately 50 kb
Elution volume 50–100 µl
Preparation time 45–120 min/96 preps (45 min for KingFisher)
Binding capacity 0.4 μg/μl beads

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
744210.1 NucleoMag® Pathogen 1 x 96 Preps $381.00
744210.4 NucleoMag® Pathogen 4 x 96 Preps $1,318.00
 

 

 
 

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