Clontech
login | register | order now | view cart | feedback
Products
Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Total_RNA_from_Cells_and_Tissues >  XS_Spin_Columns

NucleoSpin RNA XS—Complete Kit for Isolation & Purification of Total RNA from Extremely Small Samples

NucleoSpin RNA XS allows the isolation of highly concentrated, highly pure total RNA from very dilute or low-volume tissue and cell samples without the use of organic compounds (e.g., Trizol).

New Column Design

The innovative funnel-shaped thrust ring allows standard miniprep spin columns to hold a silica membrane of very small diameter. The small surface of the NucleoSpin RNA XS membrane allows efficient elution in only 5–20 µl, resulting in the highest RNA concentrations. The purified RNA is ready for all typical downstream applications.

Procedure

Cells are lysed by incubation in the lysis buffer (high concentration of chaotropic salts). This lysis buffer immediately inactivates RNases—which are present in virtually all biological materials—and creates appropriate binding conditions which favor adsorption of RNA to the silica membrane. After lysis, homogenization and reduction of viscosity are achieved by filtration with NucleoSpin Filter units (shredders). Residual genomic DNA is efficiently removed by on-column digestion with rDNase, provided with the kit. Total RNA is eluted in RNase-free water. The low elution volume results in concentrated RNA with typical RIN (RNA Integrity Numbers) >9 (depending on sample quality).

At-A-Glance Documents Images & Data Resources

Features

  • Purify RNA from a single cell, or 0.1 mg of tissue
  • Obtain highly concentrated RNA for sensitive downstream applications—elute in as little as 5 µl
  • Complete kit with rDNase included for optimal on-column genomic DNA removal
  • Filters included for homogenization and reducing lysate viscosity
  • Yields high-quality, ready-to-use RNA for RT-PCR and other sensitive applications

Applications

Complete kit for isolation and purification of total RNA from cultured cells, tissue, cryosections, laser-captured cells, small amounts of plant material, and samples stored in RNAlater.

Purified RNA is suitable for:

  • Real-time RT-PCR
  • Northern blotting
  • Primer extension
  • Array technology
  • RNase protection assays

Product Overview

Technology Silica membrane
Format XS spin columns
Starting Material 1–100,000 cultured cells
<5 mg tissue
Fragment Size >200 bp
Typical Yield 0.1–1,500 ng
A260/280 1.90–2.10
Elution Volume 5–20 µl
Preparation Time 40 min/12 preps

 
 
Products
Offers Cat. # Product Package Size Price # of Units Select
  740902.250 NucleoSpin® RNA XS 250 Preps $1,507.00
740902.50 NucleoSpin® RNA XS 50 Preps $341.00
  740902.10 NucleoSpin® RNA XS 10 Preps $76.00
 

 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions